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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Characterization of embryonic stem cell-differentiated fibroblasts as mesenchymal stem cells with robust expansion capacity and attenuated innate immunity

Fig. 5

mESC-FBs do not respond to LPS. a BM-MSCs (upper panels) and mESC-FBs (bottom panels) were treated with LPS for 60 min or TNFα for 15 min, or left untreated (Con). The cellular location of NFκB was detected with an antibody against NFκB under a fluorescence microscope. Scale bar = 50 μm. b Cells were treated with LPS for the indicated time. The expression of TNFα, ICAM1, and IL6 was determined by RT-qPCR. Con represents cells without any treatment. Values are mean ± SD of samples from three independent experiments. *p < 0.05, compared with respective controls (Con)

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Stem Cell Research & Therapy

ISSN: 1757-6512