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Table 1 Research design for optimization of urothelially differentiated human USC

From: Urothelium with barrier function differentiated from human urine-derived stem cells for potential use in urinary tract reconstruction

Group (G)

Dynamic culture

Static culture

Single seeding

Triple seeding

Single seeding

Triple seeding

G1 USC

USC (5 × 105 cells/well) as negative control

G2 UC

UC (5 × 105 cells/well) cultured in KSFM as positive control

G3 USC + UC/CM

USC (5 × 105 cells/well) cultured with conditioned Media from UC culture

G4 USC + EGF

USC (5 × 105 cells/well) cultured in induce Media with EGF (30 ng/ml)

G5 USC + SMC/CM

USC (5 × 105 cells/well) cultured with EGF (30 ng/ml) and conditioned medium from SMC culture (1:1)

  1. Abbreviations: USC urine-derived stem cells, UC urothelial cells, SMC smooth muscle cells, CM conditioned medium, UC/CM urothelium-conditioned medium, SMC/CM Smooth muscle cell-conditioned medium, EGF epidermal growth factor
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Stem Cell Research & Therapy

ISSN: 1757-6512