Table 1 Trilineage differentiation scoring rubric for all lineages. Chondrogenesis rubric based on the Bern Score [15]
From: In vitro MSC function is related to clinical reaction in vivo
Lineage | Score |
|---|---|
Chondrogenesis | |
Uniformity and darkness of stain | |
No stain | 0 |
Weak staining of poorly formed matrix | 1 |
Moderately even staining | 2 |
Even dark stain | 3 |
Distance between cells/amount of matrix accumulated | |
High cell densities with no matrix between | 0 |
High cell densities with a little matrix in between | 1 |
Moderate cell density with matrix | 2 |
Low cell density with a moderate distance between | 3 |
Cell morphologies represented | |
Condensed/necrotic/pycnotic bodies | 0 |
Spindle/fibrous | 1 |
Mixed spindle/fibrous with rounded chondrogenic morphology | 2 |
Majority rounded/chondrogenic | 3 |
Adipogenesis | |
0% of cells with stain uptake | 0 |
1–25% of cells with stain uptake | 1 |
26–50% of cells with stain uptake | 2 |
51–75% of cells with stain uptake | 3 |
76–100% of cells with stain uptake | 4 |
Osteogenesis | |
0% of cells with stain uptake | 0 |
1–25% of cells with stain uptake | 1 |
26–50% of cells with stain uptake | 2 |
51–75% of cells with stain uptake | 3 |
76–100% of cells with stain uptake | 4 |