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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors

Fig. 4

Characterization of pancreatic hormone in human ESC-derived culture at day 13. a Time course culture of non-transfected and transfected cells at days 5, 9, and 13. Scale bars, 500 μm. b Time course of gene expressions. Insulin, glucagon, and somatostatin were analyzed by qRT-PCR at day 13. The Y axis indicates the relative change of mRNA expression compared with that of no transfection. Results were shown relative to the endogenous control GAPDH. *P < 0.05. Error bars indicate SEM n = 4. c Upper panels show insulin (red) and glucagon (green) immunostainings and a DAPI staining (blue) at day 13. Scale bars, 200 μm. Lower panels show insulin (red), glucagon (green), and somatostatin (blue) immunostainings and a DAPI staining (gray) at day 13. Monohormonal insulin-positive cells: white arrow heads. Scale bars, 15 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512