Fig. 4

NF inclusions in ALS iPSC-derived neurons. a Immunofluorescent images of NF-H, NF-M, and NF-L in CHAT+ MNs. NF staining in the insets is magnified on the right panel. Arrows indicate NF inclusions in the cell body; arrowheads indicate NF inclusions in neurites. Scale bar = 50 μm. b Quantification of NF inclusion-containing cell bodies and neurites in MNs and non-MNs at days 4, 7, and 10 after plating. *p < 0.05. c EM showing NF arrangement in cell body and neuritis of MN cultures. Scale bar = 2 μm. d Immunofluorescent images of NF-H, NF-M, and NF-L in non-MNs. Scale bar = 50 μm