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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Tropism of mesenchymal stem cell toward CD133+ stem cell of glioblastoma in vitro and promote tumor proliferation in vivo

Fig. 2

a, b Culture of MSCs with approximately 80–90% confluence. Magnification: ×100. c Induction of adipocyte-like phenotype by oil red stain. d Induction of osteogenic-like by Alizarin red stain. c, d′ Undifferentiated control for adipogenic and osteogenic differentiation, respectively. e, f Fluorescence detection of MION-Rh labeling in the MSCs. Magnification: a, b, d, e ×100; c, f ×400. g Graphs summarize FACS analysis of MSC expression of cell markers: 73.2% of MSCs reacted with the anti-CD73 antibody; 98.7% of MSCs reacted with the anti-CD44 antibody; 95.9% of MSCs reacted with the anti-CXCR4 and anti-CCR2 antibodies and there was low or no expression of CD31 and CD45 markers. H RT-PCR analysis of CXCR4 and CCR2 mRNA levels expressed in MSCs (triplicate samples). FACS and RT-PCR analysis are representative of all collected MSCs samples

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Stem Cell Research & Therapy

ISSN: 1757-6512