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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: In vitro and in vivo potentialities for cartilage repair from human advanced knee osteoarthritis synovial fluid-derived mesenchymal stem cells

Fig. 1

Immunophenotype and trilineage differentiation of fibroblastic cells derived from advanced OA synovial fluid. a Surface marker expression of human MSCs derived from advanced OA synovial fluid at passage 2. Each histogram is a representative result of three SF-MSC samples. The white histogram represents negative control response, and black histogram is for the samples. Results showed negativity for CD34, CD45, and HLA-DR and positivity for MSCs markers CD90, CD105, and CD73. b Chondrogenic, osteogenic, and adipogenic potentials of SF-MSCs. The chondrogenic differentiation of SF-MSCs was assessed by alcian blue staining on pellet cultured during 28 days in chondrogenic medium (TGF-β1). Cells were cultured in osteogenic medium during 14 days, and calcium mineralization was evaluated by Alizarin Red S staining. Lipid droplets were observed in SF-MSCs cultured in adipogenic induction medium for 28 days with Red Oil staining. A representative example for two samples is shown. Scale bars 200 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512