Fig. 1
From: miR-181b/Notch2 overcome chemoresistance by regulating cancer stem cell-like properties in NSCLC
Reduced miR-181b expression promotes CSC properties in NSCLC. A549/DDP and A549 cells were transfected with miR-181b mimics, miR-181b inhibitors, or the control. a, b The number of tumourspheres were counted, and the morphology was observed under a light microscope. c CD133+ cells were analysed in A549/DDP and A549 spheres by flow cytometry. d The mRNA levels of KLF4, SOX2, NANOG, CD133, and ALDH were measured by qPCR. Bars represent 200 μm for the low-power lens and 50 μm for the high-power lens. Data are presented as the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001