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Table 1 Experimental details of studies, mouse strains, cell types, route of administration, dose and number of animals studied

From: Non-invasive imaging reveals conditions that impact distribution and persistence of cells after in vivo administration

Study Mouse strain Cell type Route Dose Number of animals
BLI short-term biodistribution (and MSOT for RAW macrophages) BALB/c mMSC IV and IC 1.0 × 106 Minimum n = 3 for each cell type and administration route
BALB/c mKSC IV and IC 1.0 × 106
BALB/c hUC-MSC IV and IC 1.0 × 106
BALB/c hBM-MSC IV and IC 5.0 × 105
BALB/c hKC IV and IC 0.3 × 105
BALB/c Macrophages IV and IC 1.0 × 107
BLI long-term biodistribution BALB/c SCID mMSC IV 1.0 × 106 n = 3
BALB/c SCID mMSC IC 1.0 × 106 n = 5
BALB/c mMSC IC 1.0 × 106 n = 4
FVB mMSC IC 1.0 × 106 n = 4
MF1 mMSC IC 1.0 × 106 n = 4
BALB/c SCID hUC-MSC IV 1.0 × 106 n = 13
BALB/c SCID hUC-MSC IC 1.0 × 106 n = 3
BALB/c SCID hUC-MSC IV 5.0 × 105 n = 6
BALB/c SCID hUC-MSC IC 5.0 × 105 n = 14
BALB/c SCID hBM-MSC IV 5.0 × 105 n = 6
BALB/c SCID hBM-MSC IC 5.0 × 105 n = 6
MRI cell tracking BALB/c mMSC IV 1.0 × 106 n = 2
BALB/c mMSC IC 1.0 × 106 n = 7
  1. Cell numbers for IC administration had to be individually optimised for each cell type used since mice responded severely to higher numbers of some of the cell types after injection into the left ventricle. Administered cell numbers caused no health problems after IV injection in any of the cell types used