Fig. 7

In vivo bone regeneration assay transplanted with SCAP and SCAP-based spherical calcified constructs. a, b SCAP were pretreated with or without LY294402 (50 μM) and rapamycin (100 nM) and were transplanted with hydroxyapatite/tricalcium carriers (HA) into the jawbone cavity of immunocompetent mice. Representative images of the jawbone cavity (BC) by hematoxylin and eosin staining. Intact, jawbone cavity transplanted with non-pretreated SCAP (a). Histological findings of jawbone cavity transplanted with SCAP-based spherical calcified constructs (b). c, d SCAP-based spheroids were pretreated with or without LY294402 (50 μM) and rapamycin (100 nM) and were cultured under osteogenic/dentinogenic condition for four weeks. The fabricated scaffold-free SCAP-based spherical calcified constructs were transplanted into the jawbone cavity of immunocompetent mice. Representative images of the jawbone cavity (BC) by hematoxylin and eosin staining. Intact, jawbone cavity transplanted with non-pretreated SCAP-based spheroids (c). Histological findings of jawbone cavity transplanted with SCAP-based spherical calcified constructs (d). a, c CT, connective tissue. Yellow-colored dot lines, original wall of bone cavity