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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Overexpression of apelin in Wharton’ jelly mesenchymal stem cell reverses insulin resistance and promotes pancreatic β cell proliferation in type 2 diabetic rats

Fig. 2

Transduction of WJ-MSCs with lentiviral vector particles for apelin expression. a, b Expressing of EGFP in transduced WJ-MSCs with MOI of 20 and 50 under fluorescence microscopy. c Analysis of EGFP fluorescence by flow cytometry at 48 h after transduction. The upper panel represents the nontransduced WJ-MSCs. d Apelin expression was detected by flow cytometry analysis using monoclonal anti-apelin antibody at 48 h after transduction. The upper channel represents the nontransduced WJ-MSCs. e Proliferation rate of the EGFP-positive transduced WJ-MSCs is significantly different among the cells with different MOI. f Cell growth of the transduced WJ-MSCs with different MOI was significantly different via MTT assay at OD 490 nm

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Stem Cell Research & Therapy

ISSN: 1757-6512