Fig. 3

Stage-specific ETV2 downregulation augments generation of mature and stable EiECs from KDR+ cells. a Schematic representation of the two-stage conversion process. b Sorted KDR+ cells cultured in endothelial maintenance medium (EMM) with or without DOX were detected by quantitative RT-PCR at different time points. Quantitative RT-PCR data are normalized to β-actin. c The final optimal scheme of direct endothelial reprogramming from transduced ETV2-hADSCs. *P < 0.05, **P < 0.01. d Immunofluorescence of endothelial cell markers CD31, VE-cad, vWF, and ac-LDL uptake were detected in EiECs; hUVECs were used as positive control. Scale bar = 50 μm