Fig. 4

Characterizations of EiECs in vitro. a Representative flow cytometric analysis of EiECs and hUVECs. b EiECs, similar to hUVECs, were able to form tubular structures on Matrigel-coated plates. c The number of branches were quantified (n = 5). Scale bar = 50 μm. d Quantitative analysis of angiocrine factor secretion among hADSCs, EiECs, and hUVECs by ELISA. e Growth curves of EiECs during long-term expansion in EMM with SB. f Cells were collected at nine passages, G banding analysis of EiECs demonstrating a normal human karyotype (44, XX). *P < 0.05, **P < 0.01