Fig. 5

In vivo differentiation studies and immunofluorescence analysis of D-P and D-P-TGF-β1 RI samples (× 40 magnification) after 1 week of transplantation. a, b Modified ADSCs were labeled with the cell track reagent CMFDA (green) and immunostained with anti-α-SMA(red) or anti-cTnT (red). Nuclei were counterstained with DAPI (blue). c, d The number of modified ADSCs expressing α-SMA and cTnT were compared (scale bar, 100 μm; values are expressed as mean ± S.D; *p < 0.05, **p < 0.01, ***p < 0.001)