Fig. 2

Intravenously infused human iPS cell-derived endothelial progenitor cells (iEPCs) were recruited to the post ischemia/reperfusion (I/R) injuries of the kidney, spleen, liver, and lung, but not the heart of nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice. a Bright-field and fluorescence images depicting undifferentiated colonies of lentivirus-GFP-transduced iPS cells on mitotically inactivated mouse embryonic fibroblasts. Scale bar = 100 μm. b Fluorescence images depicting a differentiated colony of GFP-iEPCs. Scale bar = 200 μm. c Fluorescence images depicting GFP-iEPCs recruited to the kidney, spleen, liver, and lung 48 h after intravenous infusion in mice with AKI induced by renal I/R injury. Arrows indicate GFP-iEPCs. Scale bar = 50 μm. d Bar chart denoting the percentage of recruited GFP-iEPCs per field captured at the original magnification × 400. The denominator is the sum of GFP-iEPCs in the kidney, spleen, liver, and lung. Data are presented as mean ± SEM. N = 4 per group