Fig. 3

bFGF alters expression of phosphate/pyrophosphate regulatory genes in SHEDs. a SHED cells cultured in osteogenic medium over 14 days increase ALPL mRNA (day 7; p < 0.05), with no significant alterations in b ANKH, c ENPP1, or d SLC20A1 expression. Inclusion of 10 ng/mL bFGF in osteogenic induction medium e reduces ALPL mRNA (days 3 and 7), f increases ANKH mRNA (days 1, 3, and 7), g does not significantly affect ENPP1 mRNA, and h increases SLC20A1 mRNA (day 1). Addition of 20 mM FGFR inhibitor (SU5402) inhibits bFGF-induced changes in i ALPL and j ANKH on day 7. k Addition of 20 mM SU5402 in the absence of exogenous bFGF increases ALPL (p < 0.05) but does not affect ANKH expression. The Mann-Whitney U test was employed for comparison of two groups. The Kruskal-Wallis test followed by pairwise comparison was used for comparison of three or more groups. Bars indicated the statistically significant difference. OM osteogenic induction medium (*p < 0.05)