Fig. 3

Establishment and characterization of Sirt6-null iPS-like cell line. a iPSC clones derived from wild-type and Sirt6 knockout Oct4-GFP MEF cells. iPS clones were picked and proliferated; GFP+ clones were considered as fully reprogrammed. b Immunofluorescence analysis showed the expression of SSEA-1 and Sox2 in Sirt6-null iPS-like cells were analyzed compared to wild-type iPSCs (red, antibody staining; blue, Hochest staining). c Pluripotency genes (Oct4, Sox2, Nanog, Esrrb, and Fgf4) in Sirt6-null iPS-like cells were analyzed compared to wild-type iPSCs by qRT-PCR. Data were normalized to the expression levels in wild-type control. Data represented as mean ± SD from three independent assays. d Teratoma formation in Sirt6-null iPS-like cells compared to wild-type iPSCs. H&E staining was performed after 4 weeks, and all of the above cells formed some typical three germ layers (bars indicated 100 μm). e Chimera formation in Sirt6-null iPS-like cells compared to wild-type iPSCs. Chimera as indicated by the agouti coat color; the rate of chimera formation was shown as table in the right panel