Table 1 eMSC isolation and immortalization efficiency
Oestrous cycle stage | Embryo location | Primary cultures | Immortalization efficiency | Immortalized eMSC | Oestrous stage |
|---|---|---|---|---|---|
D2-D4 early luteal | Embryo in the oviduct | 4 | 1 (25%) | 1A | 1 |
D4-D8 medium luteal | Embryo in the uterus with zona pellucid (ZP) | 4 | 0 (0%) | – | 2 |
D9-D16 late luteal | Embryo in the uterus w/o ZP | 8 | 3 (37.5%) | 3A, 3D, 3E | 3 |
D17-D21 follicular phase | Regression of corpus luteum if not embryo | 6 | 4 (66.7%) | 4B, 4C, 4D, 4H | 4 |