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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Distinct roles of Dlk1 isoforms in bi-potential differentiation of hepatic stem cells

Fig. 4

Dlk1 cells and Dlk1+ cells had similar proliferative capacity. Dlk1+ cells and Dlk1 cells derived from Dlk1+ cells were sorted using FACS. a Dlk1+ or Dlk1 cells were plated in six-well collagen-coated plates at a density of 300 cells per well. After 10–12 days of culture, the colonies were stained with crystal violet, and the number of colonies was determined. b Dlk1+ and Dlk1 cells were plated at 1 × 105 cells per well in six-well collagen-coated plates. After 48 h, BrdU was added to cells at a final concentration of 10 μmol/L for 4 h, and then, BrdU labeling was assessed via FACS, and the proportion of BrdU-positive (BrdU+) cells was calculated. c Dlk1+ and Dlk1 cells were seeded at a density of 1 × 103 cells per well in 96-well plates. Cell growth was measured daily using CCK8 assays. d The expression levels of cyclin D1, cyclin E1, cyclin A2, and cyclin B1 in Dlk1+ cells and Dlk1 cells were measured via quantitative real-time PCR. Representative images from one of three experiments are shown, and the data are shown as the mean ± SEM of three independent experiments (Mann-Whitney test in a and b, one-way ANOVA with post Dunn’s multiple comparisons test in c, two-way ANOVA with post Bonferroni’s multiple comparisons test in d). n.s. not significant

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