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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Distinct roles of Dlk1 isoforms in bi-potential differentiation of hepatic stem cells

Fig. 5

Dlk1 cells appeared to be cholangiocyte progenitor-like cells. Dlk1+ cells and Dlk1 cells derived from Dlk1+ cells were sorted using FACS. a, b Dlk1+ cells and Dlk1 cells were induced for hepatic differentiation with Matrigel and OSM. Phase-contrast images presented the morphologies of the cultured Dlk1+ cells and Dlk1 cells on Matrigel after 8 days (a). Arrows indicate differentiated cells. b Cells engaged in hepatic differentiation for the indicated time were collected, and the expression of the hepatic maturation markers ALB, G6P, and TAT was determined on day 2 and TO expression was measured on day 8 via quantitative real-time PCR. c, d Dlk1+cells and Dlk1 cells were induced for cholangiocytic differentiation in collagen gel-embedded cultures. Phase-contrast images show representative views of branching structures derived from Dlk1 cells compared with Dlk1+ cells (c). Arrows indicate bile duct-like branching structures. Cells engaged in cholangiocytic differentiation for the indicated times were collected, and the expression of the cholangiocyte marker CK19 was determined with quantitative real-time PCR (d). Representative images from one of three experiments are shown, and the data are shown as the mean ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 (Mann-Whitney test and two-way ANOVA with post Bonferroni’s multiple comparisons test)

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