Fig. 7

bFGF played a part in inducing the loss of Dlk1^M. a The expression levels of α-SMA, Vimentin, and OPN in Dlk1⁺ and Dlk1⁻ cells were measured via quantitative real-time PCR. b Dlk1⁺ cells were plated in six-well collagen I-coated plates at 1 × 10⁵ cells per well and cultured with different sets of growth factors. After 6 days, the expression of Dlk1 was measured via FACS. c Dlk1⁺ cells were induced to cholangiocytic differentiation with the addition of increasing amounts of bFGF. After 6 days, the expression level of CK19 was analyzed via quantitative real-time PCR. d The autophagy marker LC3II (green) was detected in Dlk1⁺ and Dlk1⁻ cells using immunofluorescence. e The expression level of LC3I/II in Dlk1⁺ and Dlk1⁻ cells was determined by western blot. f Dlk1⁺ cells were plated in six-well collagen I-coated plates and cultured with different sets of growth factors for 6 days. The expression level of LC3I/II was determined by western blot. Representative images from one of three experiments are shown, and the data are shown as the mean ± SEM of three independent experiments. *P < 0.05 (two-way ANOVA with post Bonferroni’s multiple comparisons test in a and Mann-Whitney test in c)