Fig. 1

Suppression of Ell3 expression induces stem cell senescence. a Quantitative reverse transcription PCR (qRT-PCR) analysis was performed on ADSCs and BM-MSCs at the indicated culture passage. The numbers of b ADSCs and ES-MSCs as well as those of c MCF7 and MCF10A cells transfected with siNS or siEll3 were counted on the indicated days after transfection. Cell cycle analysis of d ADSCs and BM-MSCs as well as e MCF7 and MCF10A cells transfected with siNS or siEll3 was performed by FACS 48 h after siRNA transfection (left panel). Quantitation of the cell cycle analysis results is presented as a graph (right panel). f The mitochondrial membrane potentials of ADSCs, BM-MSCs, MCF7 cells, and MCF10A cells transfected with siNS or siEll3 were evaluated by JC-1 staining (left) and flow cytometry analysis (right). Scale bar 25 μm. g β-gal staining was performed with ADSCs and BM-MSCs transfected with siNS or siEll3. β-gal (+) cells were imaged under a light microscope (left) and quantified (right). Scale bar 20 μm. The experiments were repeated three times independently, and the results presented as bars represent the mean ± s.d. Abbreviations: siNS, nonspecific siRNA; siEll3, siRNA targeting Ell3; ADSCs, adipose stem cells; BM-MSCs, bone marrow-derived mesenchymal stem cells