Fig. 3

Long-term monitoring cell engraftment in mice transplanted at neonatal age. a Representative bioluminescence image of NSG mice transplanted with ECFCs only, PMSCs only or ECFCs plus PMSCs at 3–5 days after birth over 26 weeks post-transplantation. b Quantitative analysis of the bioluminescence signal intensity in these mice over time. c Real-time PCR analysis of different transplantation groups at 26 weeks post-transplantation for the presence of human cells using human-specific ERV3 and mouse GAPDH primers. (D) Comparison of cell retention at 4 to 24 weeks of post-transplantation of mice between transplanted cells at the neonatal period and adult. Data were expressed as mean ± SEM. *p < 0.05, **p < 0.01, ##p < 0.01, ###p < 0.001. n = 5