Fig. 1

Morphology of the testis from OA and SCOS patients and identifications of isolated human Sertoli cells. a H&E staining illustrated the morphology of testicular tissues from OA (left panel) and SCOS patients (right panel) (scale bar = 20 μm). b RT-PCR showed that the human Sertoli cells isolated from OA (1) and SCOS (2) patients’ testes expressed transcripts of GATA4, ABP, WT1, and FSHR. ACTB (actin beta) was used as a loading control of total RNA. c Immunohistochemistry reveals that GATA4 is expressed in human Sertoli cells isolated from OA and SCOS patients’ testes (scale bar = 10 μm). Note: OA obstructive azoospermia, SCOS Sertoli cell-only syndrome, SC Sertoli cell