Fig. 3From: Low-intensity pulsed ultrasound promotes chondrogenesis of mesenchymal stem cells via regulation of autophagyEffects of LIPUS on the chondrogenesis of MSCs. The MSCs were cultured in basic medium or chondrogenic medium for 10 days before analyses. a Representative images of ICC showing COL2+ cells (upper panel), and toluidine blue-staining showing AGG (lower panel) in differentiating MSCs stimulated with varying intensities of LIPUS; scale bars = 100 μm. b–d Bar graphs showing relative levels of COL2 (b), AGG (c), and SOX9 (d) mRNA in LIPUS-stimulated and unstimulated MSCs. The values are the mean ± SD of triplicate experiments; *P < 0.05Back to article page