Fig. 3

U251 cell-derived exosomes contribute to the promotion of the migration and invasion of hBMSCs. a The movement of hBMSCs was observed by wound healing assay at 0 h and 48 h after exosomes treatment. b The wound closure rate of hBMSCs was quantified by measuring wound closure areas at 0 h and 48 h (*P < 0.05, **P < 0.01). c The migration and invasion abilities of hBMSCs were examined by Transwell assay after treatment with U251 cell-derived exosomes for 48 h. The photos represented cell invasion into the underside of the Transwell membrane under a microscope at × 200 magnification field. d The hBMSC number that crossed the Transwell chamber of each group was counted and averaged after treatment with U251 cell-derived exosomes for 48 h (*P < 0.05, **P < 0.01). e hBMSCs were treated with 300 μg/mL U251 cell-derived exosomes for 48 h. MMP-2 and MMP-9 proteins in hBMSCs were detected by Western blotting. The ratios of MMP-2 and MMP-9 were relative to GAPDH and standardized to the control group (*P < 0.05). f The mRNA expression levels of MMP-2 and MMP-9 in hBMSCs treated with U251 cell-derived exosomes for 48 h (*P < 0.05)