Fig. 4

Tumor-associated factors were induced or increased in hBMSCs by treatment with exosomes from U251 cells for 48 h. a Fluorescence images showing that the hBMSCs express the glioma-related marker GFAP after treatment with 300 μg/mL U251 cell-derived exosomes (× 200). b The protein levels of GFAP, CD133, and Nestin were examined by Western blotting after treatment with 150, 300, and 600 μg/mL U251 cell-derived exosomes. c Bar graphs showing the relative protein expression level of GFAP, CD133, and Nestin. The protein levels of GAPDH served as loading controls (*P < 0.05,**P < 0.01). d The mRNA expressions of GFAP, CD133, and Nestin. GAPDH was used as the endogenous control (*P < 0.05)