Fig. 7

ASC-MVs enhanced neovascularization and cellular proliferation in vivo as evidenced by immunofluorescence analysis. a Ki-67 (red color) immunofluorescence staining of wound sections treated with either 50 μg ASC-MVs or an equal volume of PBS at day 13 post-wounding. b Qualification of the ratio of Ki67+ cells in wound beds. c Immunofluorescent triple staining of wound sections given the above treatments at day 13 post-wounding. Smooth muscle cells (a-SMA), endothelial cells (CD34), and cell nuclei (DAPI) were stained with green, red, and blue colors. Newly formed vessels express CD34. Mature vessels express both CD34 and a-SMA. d Enumeration of newly formed vessels stained with red color. e Enumeration of mature vessels co-stained with red and green colors. Scar bar, 100 μm. N = 7. **p < 0.01, ***p < 0.001, ****p < 0.0001