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Table 1 The sequences of primers of qPCR

From: Continuous exposure of isoprenaline inhibits myoblast differentiation and fusion through PKA/ERK1/2-FOXO1 signaling pathway

Gene Forward Reverse
MyoG 5’-GAGACATCCCCCTATTTCTACCA-3’ 5’-GCTCAGTCCGCTCATAGCC-3’
MyoD1 5’-CCACTCCGGGACATAGACTTG-3’ 5’-AAAAGCGCAGGTCTGGTGAG-3’
MyHC1 5’-CAAGCAGCAGTTGGATGAGCGACT-3’ 5’-TCCTCCAGCTCCTCGATGCGT-3’
MyHC2a 5’-AGAGGACGACTGCAGACCGAAT-3’ 5’-GAGTGAATGCTTGCTTCCCCCTTG-3’
MyHC2b 5’-ACGCTTGCACACAGAGTCAG-3’ 5’-CTTGGACTCTTCCTCTAGCTGCC-3’
MyHC2x 5’-ACCAAGGAGGAGGAACAGCAGC-3’ 5’-GAATGCCTGTTTGCCCCTGGAG-3’
GAPDH 5’-ATGACTCCACTCACGGCAAA-3’ 5’-ATGATGACCCTTTTGGCTCC-3’
  1. qPCRs were performed to identify the traits of satellite cell differentiation and muscle fibers by using the specific primers of satellite cell differentiation markers including MyoD and MyoG, type I muscle fiber makers like MyHC1, and type II muscle fiber makers such as MyHC2a, MyHC2b, and MyHC2X. GAPDH as an internal control