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Table 1 The sequences of primers of qPCR

From: Continuous exposure of isoprenaline inhibits myoblast differentiation and fusion through PKA/ERK1/2-FOXO1 signaling pathway

Gene

Forward

Reverse

MyoG

5’-GAGACATCCCCCTATTTCTACCA-3’

5’-GCTCAGTCCGCTCATAGCC-3’

MyoD1

5’-CCACTCCGGGACATAGACTTG-3’

5’-AAAAGCGCAGGTCTGGTGAG-3’

MyHC1

5’-CAAGCAGCAGTTGGATGAGCGACT-3’

5’-TCCTCCAGCTCCTCGATGCGT-3’

MyHC2a

5’-AGAGGACGACTGCAGACCGAAT-3’

5’-GAGTGAATGCTTGCTTCCCCCTTG-3’

MyHC2b

5’-ACGCTTGCACACAGAGTCAG-3’

5’-CTTGGACTCTTCCTCTAGCTGCC-3’

MyHC2x

5’-ACCAAGGAGGAGGAACAGCAGC-3’

5’-GAATGCCTGTTTGCCCCTGGAG-3’

GAPDH

5’-ATGACTCCACTCACGGCAAA-3’

5’-ATGATGACCCTTTTGGCTCC-3’

  1. qPCRs were performed to identify the traits of satellite cell differentiation and muscle fibers by using the specific primers of satellite cell differentiation markers including MyoD and MyoG, type I muscle fiber makers like MyHC1, and type II muscle fiber makers such as MyHC2a, MyHC2b, and MyHC2X. GAPDH as an internal control