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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Long noncoding RNA LINC00707 sponges miR-370-3p to promote osteogenesis of human bone marrow-derived mesenchymal stem cells through upregulating WNT2B

Fig. 3

LINC00707 stimulates osteogenic differentiation of HBMSCs in vitro and in vivo. a Efficiency of interference and overexpression of LINC00707 in HBMSCs by qRT-PCR. b mRNA levels of RUNX2, OCN, and ALP in transfected HBMSCs after 7 days of osteogenic induction by qRT-PCR. c Western blotting analysis of protein level of RUNX2 in transfected HBMSCs after 7 days osteogenic induction. d Relative activity of ALP after interference or overexpression of LINC00707 in transfected HBMSCs after 7 days osteogenic induction. e ARS staining and ALP staining after interference or overexpression of LINC00707 in transfected HBMSCs after 7 days osteogenic induction. f Transfected HBMSCs after 7 days osteogenic differentiation loaded on HA-TCP were transplanted into the dorsal region of NOD/SCID mice. Xenografts were removed and stained with HE and Masson’s Trichrome stain, respectively. Data represent the mean ± S.D. *P<0.05, **P<0.01

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