Fig. 4

PTGS1 affects osteogenesis of ASCs in vivo. a PTGS1 knockdown promoted ASC-mediated bone-like tissue formation in vivo, as measured by HE staining, Masson’s trichrome staining, and immunohistochemistry staining of OCN in PTGS1sh and NC groups. Scale bar represents 50 μm. b Quantitative measurements of bone-like tissues showed that the area of bone formation was notably increased in PTGS1 knockdown cells compared with NC cells. c, d PTGS1 overexpression impaired the osteogenic differentiation in ASCs, as shown by HE staining, Masson’s trichrome staining, immunohistochemistry staining of OCN, and quantitative measurements of bone-like tissue. Scale bar represents 50 μm. All data are shown as the mean ± SD, n = 3. **P < 0.01