Fig. 6

PTGS1 promotes the translocation of p65 from the cytoplasm to the nucleus. a The protein levels of p-IκBα, total IκBα, p-p65, and total p65 were measured by Western blot in the absence and presence of TNFα for 0.5 h. b The cellular localization of endogenous p65 was observed by confocal microscopy in both control and PTGS1-overexpressing cells with or without TNFα treatment. Scale bars, 100 μm. c The nuclear and cytoplasmic protein levels of p65, tubulin, and PCAF were measured by Western blot after subcellular fractionation from EGFP-PTGS1 and vector groups untreated or treated with TNFα for 0.5 h. GAPDH was used as an internal control. All data are shown as the mean ± SD, n = 3