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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Efficient generation of male germ-like cells derived during co-culturing of adipose-derived mesenchymal stem cells with Sertoli cells under retinoic acid and testosterone induction

Fig. 8

Expression of male GC-related markers in 2.5 and 5 μM SB431542-treated ADMSCs and untreated cells on 21d. a The mRNA expression of GC-related markers Stella, DDX4, Dazl, PGP9.5, and ITGα6 in ADMSCs were evaluated by qRT-PCR. b The protein expression of phosphorylation of AKT (p-AKT) and total AKT in ADMSCs was significantly downregulated after treating with 5 μM LY294002 for 21 days than that of untreated cells. c The protein expression of GC-related markers in ADMSCs were examined by western blot. Different letters on the bar graph indicate significant differences between the groups at the 0.05 level (n = 3)

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Stem Cell Research & Therapy

ISSN: 1757-6512