Fig. 5

C-MYC overexpression inhibited chondrogenesis and correlated with increase of hypertrophy. C-MYC-MSC or GFP-MSC were expanded to passage 4, subjected to chondrogenic differentiation for 6 weeks, and induction of chondrogenic markers was evaluated. a Representative images for Safranin O staining in MSC from two selected donors (n = 4), as indicated; scale bar, 200 μm. b Proteoglycan deposition assessed with DMMB assay; n = 3. c DNA content quantification done with PicoGreen kit; n = 3. d SOX9 mRNA expression measured by qRT-PCR, in relation to GFP control; n = 4. e COL2A1 mRNA expression and the ratio of COL10A1 to COL2A1 mRNAs were analyzed by qRT-PCR; n = 4. *P < 0.05