Fig. 2

Identification of the hADSC-derived exosomes and their uptake by HUVECs. Identification and characterization of hADSC-derived exosomes by TEM (a), NanoSight (b), and Western blot detection of exosome surface markers CD9 and flotillin (c). Bar = 100 nm. d Immunofluorescence staining identification of HUVECs by an endothelial cell marker, vWF. Bar = 50 μm. e Representative photos of the HUVEC uptake of PKH26 labeled exosomes (10 μg/ml) at 3 h, 6 h, 12 h, and 21 h. Bar = 10 μm