Fig. 4
From: Impact of HOXB7 overexpression on human adipose-derived mesenchymal progenitors
HOXB7 does not alter AD-MSC adipogenic differentiation but enhance osteogenic potential and favors the chondrogenic potential of AD-MSC. a Adipogenic differentiation after 10 days of induction visualized by Oil Red O staining in AD-MSC-HOXB7 and AD-MSC-GFP; uninduced controls (inset). Scale bar 100 μm. (b) Histograms show the mRNA expression of the adipogenic markers PPAR-γ and LPL after induction in AD-MSC-GFP and AD-MSC-HOXB7. Data are the mean of three biological samples, *p value = 4.90E-06. c Von Kossa staining after osteogenic induction of AD-MSC-GFP and AD-MSC-HOXB7 (uninduced control, inset). Scale bar 100 μm. ImageJ quantification of the positive stained area on the right; *p value = 2.3E−32 HOXB7 versus GFP after 10 days of induction, **p value = 1.9E−61, and ***p value = 2.4E−80. d mRNA expression of osteogenic markers ALP (*p value = 0.02), COL1A1, and DCN. Histograms show the relative expression of the markers in AD-MSC-GFP and AD-MSC-HOXB7 after induction. Data are the mean of three biological samples. e Chondrogenic differentiation after 21 days of induction visualized by Safranin O/Fast Green staining in AD-MSC-GFP and AD-MSC-HOXB7; uninduced controls (inset). Scale bar 100 μm. f Histograms show the mRNA expression of adipogenic markers COL2A1 and SOX9 in AD-MSC-GFP and AD-MSC-HOXB7 after induction. Data are the mean of three biological samples, *p value = 0.01 and 0.00008, respectively