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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Bone-derived Nestin-positive mesenchymal stem cells improve cardiac function via recruiting cardiac endothelial cells after myocardial infarction

Fig. 4

Effects of transplanted Nestin+ and Nestin BMSCs on cardiac remodeling after MI. a HE staining of hearts showing inflammatory cells infiltration in different groups (n = 3 for each group). Scale bar, 100 μm. b The number of inflammatory cells showed no difference among the three groups. Cells were calculated from five randomly different fields under microscopic observation (× 400). Data are shown as the mean ± SEM of triplicate experiments. c TUNEL staining of infarcted zone showing the changes in apoptosis cells in different groups (n = 3). Scale bar, 20 μm. d The number of apoptosis cells showing the reduction of TUNEL-positive cells in Nestin+ BMSC group. Data are shown as the mean ± SEM from five different fields. e Immunofluorescence staining of cells positive for Sca-1 (green), CM-Dil (red), and DAPI (blue) in the post-MI myocardium after Nestin+ or Nestin BMSC transplantation (n = 3 for each group). Scale bar, 50 μm. f Percentage of Sca-1-positive cells showing the accumulation of Sca-1+ cells in Nestin+ BMSC group. Data are shown as the mean ± SEM from five different fields. *p < 0.05, **p < 0.01, ****p < 0.0001. g The percentage of CD31 expression in Sca-1+ cells, which were isolated from the infarcted heart in the Nestin+ BMSC-treated group

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