Table 1 Primary, secondary, and pre-conjugated antibodies
From: Mesenchymal stem cells transplanted into spinal cord injury adopt immune cell-like characteristics
Antibody type | Host | Target | Fluorochrome | Manufacturer (ID) | Dilution |
|---|---|---|---|---|---|
Primary | Mouse | NeuN | NA | Merck Millipore (MAB377) | 1:1000 |
Mouse | TuJ1 | NA | Merck Millipore (MAB1637) | 1:100 | |
Rabbit | GFAP | NA | Dako (Z0334) | 1:1000 | |
Rat | MHC-II | NA | Abcam (ab25333) | 1:200 | |
Rabbit | Caspase-3 | NA | Abcam (ab13847) | 1:100 | |
Secondary | Goat | Mouse | Alexa 488 | Life Technologies (A11001) | 1:500 |
Goat | Rabbit | Alexa 488 | Life Technologies (A11008) | 1:400 | |
Pre-conjugated | NA | CD29 | APC | eBioscience (17-0291-80) | 1 μg per 1 × 106 cells |
NA | Sca1 | FITC | BD (561077) | ||
NA | CD45 | v450 | BD (560697) | ||
NA | MHC-I | FITC | BioLegend (125508) | ||
NA | MHC-II | FITC | BioLegend (107606) |