Fig. 5

P27 siRNAs prevent the entrance of NPSCs into quiescence. a The quantitative PCR analysis of P27 mRNA expression was performed using NPSCs transfected with P27 siRNA (siP27) and a scrambled negative control siRNA (siCTR). b Representative Western blot analysis showed a reduction of P27 following the transfection of NPSCs with p27 siRNA. c The transfected NPSCs were stained with Pyronin Y and Hoechst 33342 and analysed by flow cytometry under normal and serum-starved conditions. d Detection of EdU incorporation in transfected NPSCs under both conditions. Data are expressed as the mean ± standard deviation, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. NC normal culture condition, SS serum starvation condition