Fig. 2

Characterization of adipocyte exosomes. a A representative electron microscopy image of adipocyte exosomes. Scale bar = 200 nm. b NTA analysis for the nanoparticle size distribution of adipocyte exosomes. c Western blot analysis of exosome marker CD63, TSG101, and cell-specific marker calnexin. Loaded protein for exosome 1 was 20 μg and exosome 2, 10 μg. d Breast cancer cells MCF7 were incubated with 200 μg/mL Dil-labeled adipocyte exosomes for the indicated times, and internalization of exosomes was determined by fluorescence microscopy. Scale bar = 100 μm