Fig. 3

IL-33 regulates HSC proliferation after irradiation. a Numbers of total cells and CFC at 72 h in irradiated bone marrow Lin⁻c-Kit⁺ cells cultured with either TSF or TSF plus IL-33. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. b Representative BrdU incorporation in bone marrow KSL cells in vivo at day 7 after 600-cGy TBI and treatment with either PBS or IL-33 (left). Right, numbers indicate the percentage of BrdU⁺ cells within the total bone marrow KSL population. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. c Left, percentage phosphorylated AKT (pAKT) in bone marrow Lin⁻c-Kit⁺ cells after 200-cGy irradiation and the culture conditions are shown. Right, CFCs from bone marrow Lin⁻c-Kit⁺ cells after 200-cGy irradiation and the culture conditions are shown. *P < 0.05 for TSF compared to IL-33, ^#P < 0.01 for IL-33 compared to IL-33 plus Ly294002 (Ly29) (mean ± SEM, n = 3 in each group). d Representative FACS analysis of the cell-cycle status of bone marrow Lin⁻c-Kit⁺ cells at 72 h after irradiation and the culture conditions shown. *P < 0.05 for TSF compared to IL-33, ^#P < 0.01 for IL-33 compared to IL-33 plus Ly294002 (Ly29) (mean ± SEM, n = 3 in each group)