Fig. 5

Antigen identification of two representative surface molecules recognized by mAb OD40 and OD46. A Antigenic molecules in the immnoprecipitants of OD40 (a) and OD46 (b) were applied to western blot (IB) with anti-leprecan-1 and anti-annexin A6 antibodies, respectively. Endogenous protein amounts were indicated in input panels. 1 and 3, IP with mouse IgG; 2 and 4, IP with mAbs. B Subcellular localization of the antigenic molecules against mAbs in the odontoblast-like cells. 1, nuclei stained by DAPI; 2, treatment with the primary mAbs and FITC-labeled secondary antibody; 3, merge with 1 and 2. Bar indicates as 100 μm