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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: microRNA-301b-3p downregulation underlies a novel inhibitory role of long non-coding RNA MBNL1-AS1 in non-small cell lung cancer

Fig. 3

Elevated lncRNA MBNL1-AS1 represses A549-SP+ proliferation, migration, invasion, drug resistance, and sphere formation. a Sphere formation of A549-SP+ and A549-SP cells detected using sphere formation assay. b CD133-positive rate of A549-SP+ and A549-SP cells detected using flow cytometry. c lncRNA MBNL1-AS1 expression of A549-SP+ and A549-SP cells determined using RT-qPCR. d Localization of lncRNA MBNL1-AS1 in A549-SP+ verified using FISH. e lncRNA MBNL1-AS1 expression of A549-SP+ after lncRNA MBNL1-AS1 overexpression determined using RT-qPCR. f Proliferation of A549-SP+ after lncRNA MBNL1-AS1 overexpression detected using CCK-8. g Drug resistance of A549-SP+ against anti-tumor drugs after lncRNA MBNL1-AS1 overexpression detected using CCK-8. h Clone formation of A549-SP+after lncRNA MBNL1-AS1 overexpression detected using clone formation assay. i Cell invasion of A549-SP+ after lncRNA MBNL1-AS1 overexpression detected using transwell assay. j Cell migration of A549-SP+ after lncRNA MBNL1-AS1 overexpression detected using scratch test. k Sphere formation of A549-SP+ after lncRNA MBNL1-AS1 overexpression detected using sphere formation assay. l Protein levels of Oct4 and ABCG2 in A549-SP+ after lncRNA MBNL1-AS1 overexpression assessed using western blot analysis; *p < 0.05 vs. A549-SP or A549 cells treated with oe-lncRNA MBNL1-AS1. Measurement data were depicted as mean ± standard deviation. Comparisons between two groups were analyzed using unpaired t test. The experiment was repeated three times. Oct4, octamer 4; ABCG2, ATP-binding cassette superfamily G (White) member 2; CCK-8, cell counting kit-8; FISH, fluorescence in situ hybridization; NC, negative control

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