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Table 2 Primer sequences for RT-qPCR

From: microRNA-301b-3p downregulation underlies a novel inhibitory role of long non-coding RNA MBNL1-AS1 in non-small cell lung cancer

Genes

Primer sequences (5′-3′)

miR-301b-3p

F: CAGGTGCTCTGACGAGGTTG

R: TGGTCCCAGATGCTTTGACA

U6

F: CTCGCTTCGGCAGCACATA

R: AACGATTCACGAATTTGCGT

lncRNA MBNL1-AS1

F: CTCCCGCTTCTTCTACCGAC

R: TTGGTGCATTTTAAGGCGGC

TGFBR2

F: GTAGCTCTGATGAGTGCAATGAC

R: CAGATATGGCAACTCCCAGTG

GAPDH

F: GAAGGTGAAGGTCGGAGTC

R: GAAGATGGTGATGGGATTTC

  1. Note: RT-qPCR reverse transcription quantitative polymerase chain reaction, MBNL1-AS1 muscleblind-like 1-antisense RNA 1, miR-301b-3p, microRNA-301b-3p, GAPDH glyceraldehyde-3-phosphate dehydrogenase, lncRNA long non-coding RNA, TGFBR2 transforming growth factor beta receptor II, F forward, R reverse
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Stem Cell Research & Therapy

ISSN: 1757-6512