Fig. 2

The effects of 20 μg/ml FN and 100 μg/ml collagen IV precoating on ESCs’ adhesion, proliferation, and colony formation. a Representative pictures of ESCs’ adhesion and proliferation in 20 μg/ml FN- and 100 μg/ml collagen IV-precoated dishes. b The proliferation of ESCs in 20 μg/ml FN- and 100 μg/ml collagen IV-precoated dishes were analyzed by CCK-8. c, d Representative pictures of ESCs’ colony formation and their cloning efficiency in 20 μg/ml FN- and 100 μg/ml collagen IV-precoated dishes. e, f Representative pictures of ESCs’ scratch-wound assays and their migration rate in 20 μg/ml FN- and 100 μg/ml collagen IV-precoated dishes. g, h Representative pictures of ESCs’ purity and their rates among all the primary adherent cells in 20 μg/ml FN- and 100 μg/ml collagen IV-precoated dishes. The values were analyzed by Graph Prism 7.0. Error bars represent SEM. Student’s t test, *P < 0.05 compared with control value (n = 5). Scale bar, 200 μm