Fig. 5

Hydrogen treatment inhibited the migration, invasion, and colony-forming ability of both C6 and U87 cells. The cell migration ability was examined using a wound-healing assay after treatment with hydrogen (a). The cell invasion ability was determined using Matrigel invasion assay after treatment with hydrogen (b). The quantification of the migration and invasion assay represents three independent experiments (c). The colony-forming ability was evaluated using colony formation assay after hydrogen treatment (d). The quantification of the colony formation assay represents three independent experiments (e). **p < 0.01, ***p < 0.001 compared with the CTRL group. CTRL, control; HRM, hydrogen-rich medium