Fig. 2

Intercellular dye transfer. Fluorescent and brightfield imaging of co-cultures of unstained and stained colonic organoids, along with quantitative assessment of dye transfer. Organoids were stained with a CMFDA, b carbocyanine-based cytoplasmic membrane dye or with c an inert membrane permeable dye. Images were taken at initiation of the co-cultures as well as 24 h later. White scale bar, 50 μm