Fig. 4

Analysis for amelogenesis of DESCs in control, amelogenesis-induction medium, amelogenesis-induction medium plus TNFα, and amelogenesis-induction medium plus BMS-345541 groups. a, c The protein expressions of AMBN, MMP20, and KLK4 were significantly elevated in the amelogenesis-induction group. Compared with the amelogenesis-induction group, treatment with TNF-α in the amelogenesis-induction medium had lower protein levels, while it had higher protein levels in amelogenesis-induction medium + BMS-345541 groups at day 3 and day 7. GAPDH served as an internal control. b, d Gray level analysis of amelogenesis-related protein in different groups at day 3 and day 7. All data were presented as the means ± SD of triplicate experiments. *P < 0.05, **P < 0.01, and ***P < 0.001. AM, amelogenesis-induction medium; BMS, BMS-345541