Fig. 2

Apoptosis induced by CRAd and Adbic in prostate tumor cells. a Flow cytometry analysis by FASCcalibur. Prostate tumor cells stained with Annexin V-FITC/Propidium iodide stains after 48 h of treatment with CRAd and Adbic adenoviral vectors at 100 MOI. b Histogram showing the representative apoptosis rate in prostate tumor cells. Results showed that both adenoviral vectors significantly raised the apoptosis rate in both prostate cancer cell lines at 100 MOI. Data represent the average of the triplicate experiment (*P < 0.05). c Expression analysis of p53, Mdm2, p21, Bax, Bcl2 proteins in C4–2, and LNCaP cells lysate, performed 48 h after treatment of C4–2 and LNCaP cells with CRAd and Adbic. Thirty microgram of total protein lysate electrophoresed on each lane. Equivalent loading was verified by stripping membranes and re-probing with actin antibody