Fig. 6

Inflammatory factors regulate fibrosis in vitro. Real-time PCR analysis of α-SMA (a) and Col1a1 (b) in mouse fibroblasts after TGF-β treatment. c The protein level detection of COL1A1 after TGF-β treatment by western blot. Real-time PCR analysis of α-SMA (d) and Col1a1 (e) mRNA expression after TGF-β and TNF-α treatment. f Western blot showed COL1A1 protein expression level after TGF-β and TNF-α treatment. Real-time PCR analysis of α-SMA (g) and Col1a1 (h) after TGF-β and IL-10 treatment. i Western blot showed COL1A1 after TGF-β and IL-10 treatment. All data are presented as the mean ± SEM; one-way ANOVA; *p < 0.05, and ns, not significant